Forensics/DNA

FTA Cards (25 stuks)

Bestel

Speciality cards for space- and time-saving collection, archival storage, and isolation of genomic DNA from a variety of sample material. Originally Whatman.

  • Capture nucleic acid in one easy step - simply apply blood or
    tissue material to the card
  • Captured nucleic acid is ready for downstream applications in
    less than 30 mins.
  • Genomic DNA collected on FTA® Cards is stable for years at
    room temperature
  • Suitable for virtually any cell type
  • Indicating FTA® Cards change colour upon sample application in order to facilitate handling of colourless samples

Simply apply your sample to the FTA® Card. Liquids are pipetted or trickled, tissue samples are pressed onto the surface. Cell membranes and organelles are automatically lysed and the released nucleic acids are entrapped in the fibres of the matrix (see figure). The nucleic acids remain immobilized and are stabilized for transport, immediate processing or long-term storage. Libraries and species DNA collections can be stored simply in a folder; single DNA samples can be filed in your lab manual. FTA® Cards facilitate sample collection in remote locations and simplify sample transport, thus, you can collect samples in the field without worrying about refrigeration.
Captured nucleic acids are used for downstream experiments by simply taking a small punch from the FTA® Card, wash with FTA® Purification Reagent and rinse with TE buffer (Roti®-Stock 100 x TE Buffer, Art. No. 1052.1). DNA on the washed punch is ready for use in a variety of applications given below. Genomic DNA stored on FTA® Cards at room temperature for over 14 years (and counting) has been successfully amplified by PCR. For RNA, being more sensitive than DNA, we recommend rapid analysis after only few weeks' storage at room temperature.

FTA® Cards contain chemicals that lyse cells, denature proteins and protect nucleic acids from nucleases, oxidative and UV damage. FTA® Cards rapidly inactivate organisms, including blood borne pathogens, and prevent the growth of bacteria and other microorganisms. Each card can be used for archival storage or multiple downstream applications of 1-2 (MINI) and 1-4 (CLASSIC) independent samples.

Suitable for blood, cultivated cells, buccal cells, plant material, bacteria, microorganisms, tissue and tissue homogenisations, virions, M13 plaques and more.

Applications: storage of genomic DNA samples and libraries, collection of nucleic acid from animal tissue or cell cultures, or from plants or microorganisms during in-field-experiments.

Downstream-Experiments: e. g. PCR, real-time PCR, SNP and STR analysis, transgenetic identification, whole genome amplification, HLA typing, and genotyping in forensics, molecular biology, food and agriculture testing, drug discovery, genomics, species identification, and more.

FTA Clonesaver cards (5 stuks)

Bestel

Speciality cards for space- and time-saving archival storage and isolation of Plasmid and BAC DNA. Originally Whatman.

  • Capture DNA in one easy step by simply applying the samples (recombinant bacteria, glycerol stocks etc.) to the card
  • Captured nucleic acid is easily accessible for downstream applications
  • DNA collected on FTA® CloneSaver Cards is stable for years at
    room temperature
  • Store up to 96 clones on each card
  • Standard 96well plate format and compatible with multi-channel pipettors

Apply few µl bacterial culture, resuspended colony or glycerol stock. Cells are lysed and plasmid or BAC DNA is stabilized for long-term storage or immediate processing. Captured nucleic acids are used for downstream experiments without the need for culture regrowth and plasmid purification. Simply take a small punch from the FTA® Card and wash with TE buffer (Roti®-Stock 100x TE Buffer, Art. No. 1052.1). DNA on the washed punch is ready for elution or for direct use in PCR, transformation, amplification by rolling circle followed by sequencing, and so on.

Pipette NOT included !

FTA Purification reagent, 500ml

Bestel
Ready-to-use, for molecular biology
For purification of nucleic acids stored on FTA® Cards. Originally Whatman.
Simple and rapid purification of nucleic acids stored on FTA® Cards.
Ensures superior quality DNA for PCR or SNP analysis.
Removes heme, PCR inhibitors and other potential contaminants.

Harris Uni-Core punches, 4 stuks

Bestel

Uni-Core Punch is a disposable punch which has a polished steel tip that is case-hardened and can be sterilized. Each tip provides up to 500 punches before disposal. Uni-Core Punch is available in diameters from 1,0 to 6,0 mm for a variety of sampling needs. It is housed in a plastic handle and disposable for contamination control. Stainless steel cutting edges enable manual coring, retrieval, and controlled ejection of the FTA punch. Manual punching is preferred for laboratories with low sample volumes punching 1 to 50 samples per day.

Very easy application: By pressing the Micro tip down a tiny slice is punched from the FTA® card. This DNA containing sample is then washed and directly applied to downstream experiments.

Two cutting mats are included. Colour of the punches supplied may differ to that shown in the picture.

FTA Foam tip applicator

Bestel
Sterile. Non-abrasive foam head is same size as sample area on FTA® Cards and is, therefore, best suited for application of buccal samples. The oral mucosa is grazed with the foam head, which is then pressed onto the card field. We recommend the use of FTA® Cards with colour indicator (CLASSIC Art. No. CL 91.1 and MINI Art. No. CL94.1) for buccal and saliva samples.

FTA Pouches

Bestel

For transporting or storing FTA® Cards. Originally Whatman.

Seven laminated layers protect the card from exposure to gas or liquid contamination. Tamper-evident seal maintains sample security. Outer paper surface for labelling or writing.
CloneSaver pouches only: with additional resealable zip-lock closure.

Thermal cycler Prime

Bestel

Compact, easy-to-handle thermal cyclers for each work bench.
Made by TECHNE.

  • Licensed for PCR
  • New, upgraded generation
  • Fast heating and cooling rates
  • High temperature homogeneity
  • Simple, intuitive programming
  • 3,5´´ colour touch screen
  • USB port
  • Cost-efficient
  • 4 years warranty on cyclers and blocks

New generation of reliable, inexpensive thermal cyclers specially developed for use on the individual bench, for student's courses and schools.

In order to enable very rapid heating and cooling rates at extremely low temperature variations in the block, an advanced block algorithm has been implemented, regulating the modern Peltier technology. The mono-tube blocks were specially designed to provide optimal contact between the PCR-vials and the block bore hole and are cut, with the most modern of technologies, out of high-grade material. In most 3PRIME instruments, the heated lid can be adjusted to the height of the PCR-vials, thus optimising the temperature and volume constancy in the sample. Intuitive programming and the newly designed colour touch screen with very easy handling, enable both simple as well as complex cycle protocols to be entered very easily and quickly. All thermal cycler models have 1000 program slots available. With Gradient Thermal Cycler a temperature gradient may be added freely to every temperature step of the program. Further data may be found online in our detailed product instruction manual or by calling one of our staff on the service hotline.

Purchase of this instrument conveys a limited non-transferable immunity from suit for the purchaser's own internal research and development and applied fields other than human in vitro diagnostics under non-real-time thermal cycler patents of Applied Biosystems LLC.

Technical Data:

TypePersonal Cycler 3PRIMEStandard Cycler 3PRIMEXGradient Cycler 3PRIMEG
Sample capacity24 x 0,2 ml or
18 x 0,5 ml
48 x 0,2 ml or
30 x 0,5 ml
48 x 0,2 ml or
30 x 0,5 ml
Block temperature4 - 100 °C
Block uniformity at 55 °Cbelow ±0,3 °C
Temperature accuracy at 55 °Cbelow ±0,2 °C
Max. heating rate3,0 °C/sec
Max. cooling rate2,1 °C/sec
Peltier elements per block244
Selectable lid temperature35 °C - 115 °C (disengageable)
Heated lid pressurenoyes4
Lid adjustable to tubes withflat lidflat and domed lidsflat and domed lids
Tubessingle tubes and strips
PCR platesno48well plates*48well plates*
Max. number of programs1000
Max. number of steps per program10 stages with 10 steps each
Max. repetition of cycles99
"Hold" time1 sec - 4 h/59 min/59 sec
Increment/Decrement functionyes (temperature and time)
Ramp resolution0,1 °C/sec
Gradient temperature range--30 - 80 °C
Max. gradient range--14 °C
Min. gradient range--1 °C
Gradient calculator--yes
Oligonucleotide Tm calculatoryes (Nearest Neighbour method)
Display3,5´´ colour touch screen
Auto-restart functionyes (disengageable)
Data transferUSB port
Password protectionyes
Warranty4 years or 80,000 cycles
Dimensions in cm
(L x W X H)
35 x 21 x 18
Weight (kg)6
Voltage100-230 V, 50-60 Hz
Power (W)155
*Heating block for 48 x 0,2 ml tubes only