Standard agar medium for detection and for assaying the potency of antibiotics.Application: Use 30,5 g for 1 l agar.Antibiotic Agar No. 1 (Ph.Eur. 7,0), Penassay Seed Agar
For isolation and identification of intestinal enterococci from water samples.Application: Use 56,6 g for 1 l agar.
For isolation, cultivation and detection of haemolytic reaction of fastidious microorganisms.Application: Use 40 g for 1 l agar. If required, add 50-110 ml defibr. blood.
For cultivation of fastidious microorganisms.Application: Use 37 g for 1 l broth.
For selective enrichment and enumeration of coli bacteria in milk, water samples, faeces and other material.Application: Use 40 g for 1 l broth.Adding 40 mg/l Novobiocin (Art. No. 0247.1) during selective detection of coliform bacteria further reduces the growth of attendant flora (Alcaide et al., 1982, J. Appl. Bact. 53:143-6).
1 Escherichia coli (turbidity and gas formation (arrow))
2 Enterobacter aerogenes (turbidity)
3 Uninoculated broth
For cultivation of Brucella and other fastidious microorganisms in foods and other specimens.Application: Use 43,1 g for 1 l agar, add 50 ml defibr. sheep blood.
For cultivation of Brucella and other fastidious microorganisms.Application: Use 28,1 g for 1 l broth.
For isolation of Campylobacter jejuni, Campylobacter coli, and Campylobacter laridis in foods, and other samples.Application: Use 45,5 g for 1 l agar. After cooling add 2 vials (2x5 ml) reconstituted Campylobacter Supplement (Art. No. 4857.1).Campy-Selective-Bloodfree-Medium, Bolton-Agar, Blood-free CCDA, Modified CCDA
Selective medium for detection of Cronobacter (formerly Enterobacter sakazakii) in milk powder and powdered infant formulae. Application: Use 30.7 g for 1 l agar
Egg Yolk Emulsion is used as supplement in nutrient agars for detection of lecithinase activity in microbial colonies. Herewith, bacterial species like Bacillus cereus and Clostridium perfringens, or Staphylococcus aureus may be identified. The lecithinase activity can be noted by formation of light, sometimes whitish halos surrounding the colonies.
Application: The ISO 7932 recommends a final concentration of 10 % emulsion in the prepared medium in general, e.g. in Mannitol Egg Yolk Polymyxin Agar (Base) (Art. No. 5765.1) for examining the lecithinase activity of Bacillus cereus.
We recommend using TSC Agar (Base) (Art. No. CL51.1), Mannitol Salt Agar (Art. No. CL81.1) or Palcam Listeria Agar (Base) (Art. No. 5768.1) for base agar with the addition of 5 % emulsion.
Add after autoclaving.
Water-soluble. Produced through aqueous extraction of autolysed brewer´s yeast. High content of vitamins. Ideal, therefore, in many standard nutrient media, e.g. LB-medium as for vitamins, carbohydrates, and amino acids.
For confirmation of Pseudomonas aeruginosa in water samples.Application: Use 38 g for 990 ml agar. Add 10 ml glycerol.
For differentiation of gram-negative enterobacteria.Application: Use 52 g for 1 l agar.Double Sugar Iron Agar, KIA
1 Salmonella enteritidis 2 Uninoculated agar
For enrichment and detection of coliform bacteria in waters.Application: Use 35,6 g for 1 l broth.Flu Broth
Selective medium for detection and enumeration of Listeria monocytogenes acc. to Ottaviani and Agosti.Application: Use 70,55 g for 1 l agar. After cooling add 2 vials (10 ml reconstituted) Listeria Chromo Selective Supplement (Art. No. 2947.1) and 2 vials (10 ml each) Listeria Chromo Lipase C Supplement (Art. No. 2948.1).
Staining of species:
A: Listeria monocytogenes cyan-blue to turquois (by reaction of the β-D-glucosidase) with halo
B: Listeria innocua green to light greenish-blue
Enterococcus and E. coli inhibited
Diluent for homogenisation of food samples and for sample preparation for microbiological analysis.Application: Use 20 g for 1 l broth.
Store unopened Petrifilm™ plate pouches at a temperature of 8° C. Return unsued plates to pouch and store resealed pouches in a cool, dry place for no longer than 1 month. Expiration dates and lot numbers are noted on each package of Petrifilm™ plates.
For enumeration of the aerobic microbial flora in milk, foods and other material.Application: Use 23,5 g for 1 l agar.